Week One (23 February - 1 March)

Dry Lab: Today’s meeting was the first day of the dry lab. We introduced ourselves and made a brainstorming about our new incoming project. And discussed about having a sponsorship and a brand new logo.

 

Week Two (2 March - 8 March)

Dry Lab: We decided on detecting fake honey as our project. We started to search how to indicate the fake honey.

 

Wet Lab: Lab equipments was introduced to new members of the team.

 

Week Three (9 March - 15 March)

Dry Lab: According to our researches we considered carboxyl induce or FDCA induce promoter. We created our team in iGEM web-site.

 

Week Four (16 March - 22 March)

Dry Lab: We planned a meeting with Prof. Dr. Irfan Kandemir to find answers to our concerns about the project.

 

Wet Lab: To introduce transformation protocol to the new members, we made a demonstration.

 

Week Five (23 March - 29 March)

Dry Lab: According to our researches we eliminated enzymes especially invertase enzyme and concentration of HMF (Hydroxymethylfurfural) in honey as an indicator.

 

Wet Lab: transformation, şeniz

 

Week Six (30 March - 5 April)

Dry Lab: Also we eliminated starch, acidity of honey, metals which are present in honey, pollen spectrum in honey as an indicator. Instead we started to search for bee diseases such as Varroa and  American Foulbrood.

 

Week Seven (6 April - 12 April)

Dry Lab: As a result of researches we found that Varroa sucks bee’s hemolymph which contains juvenile hormone. Juvenile hormone is an important factor for developing and reproduction for many insects and to Varroa aswell. We thought using a similar thing to juvenile to repel the Varroa.

 

Week Eight (13 April - 19 April)

Dry Lab: We brainstormed about the things we can do for human practice.

 

Wet Lab: Made an E.coli ONC. Prepeared six 1:1 glycerol competent cells.

 

Week Nine (20 April - 26 April)

Dry Lab: As collabration, we will send the abstract of our project to other teams and we will want them to translate to their native language. Also we discussed to arrange a ‘beekeepers meeting’.

 

Wet Lab: We prepeared six 1:1 glycerol competent cells. Parts Bba_J23116, Bba_B0032 and Bba_B0010 were transformated.

 

Week Ten (27April - 3 May)

Dry Lab: We discussed the alternative pathways of the project in terms of pros and cons.

 

Week Eleven (4 May - 10 May)

Dry Lab: Team logo has been designed. Since the latest transformation plates hadn’t show any growth and according the trouble shooting we agreed on the agar which was expired or it’s concentration was wrong.

 

Wet Lab: Parts Bba_B0032, Bba_B0010, Bba_R0010 and Bba_J23116 were transformated. Plasmid isolation proceeded .To parts Bba_B0032 and Bba_B0010  streak plate made.

 

Week Twelve (11 May - 17 May)

Dry Lab: We came up with some ideas for template of the poster.

 

Wet Lab: Plasmid isolation applied to parts Bba_B0032 and Bba_B0010.

 

Week Thirteen (1 June - 7 June)

Wet Lab: PCR applied to Lac Z. Parts K517003 (pinene odor), J45700 (winter green odor) E0040, B0034, E0240 were transformated. Parts  BL21, J23116, J45700 (winter green odor), K517003 (pinene), B0010 (term) leaved for Overnight Culture. For parts R0010 and B0032 strick plate from ONC was applied. Plasmid Isolation protocol applied to parts B0032; 1 J23716; 7.1, J23716; 8.1 and R0010.

 

Week Fourteen (8 June - 14 June)

Dry Lab: We hosted  Beekeepers Meeting at our school. Prof. Dr. Ender Yarsan from Veterinary Faculty of Ankara University and 10 beekeepers attended the meeting.

 

Wet Lab: Plasmid Isolation proceeded parts on  K517003, J45700, B0010, B0034, E0240 and E0040. Ligation protocol applied to parts B0034 and K51700. Digestion protocol applied to parts

B0034, K51700, J45700 and K517003.

 

Week Fifteen (15 June-21 June)

Wet Lab: Plasmid isolation proceeded on L1, we made  the gel control of L1. Digestion applied on M(L1) and E0240. Ligation of M(L1) and EO240 and then contolled by gel electrophoresis.

Made ligation of L1 again.

 

Week Sixteen (29 June- 5 July)

Wet Lab: Parts left for overnight culture of L1,BL21, J23116,K51700 and E0240 for two times. We made the characterization of parts for METU team. Made gel extraction of parts, J23116 and K517003. Prepared agar plates, empty, Chl. , Amp.  and Kona.  and autocloved.  Plasmid isolation of L1 ‘s ONC and the digestion of K517003 and E0240 and gel control of the L. Prepared alcohol (70%).  Also Autoclaved LB Broth.

 

Week Seventeen (6 July - 12 July)

Wet Lab: Made the gel extraction from digested parts, J23116,K517003,E0240 ,the plasmid isolation of

K517003, E0240, I74211 for two times,J23116 for one time  and digestion of K517003 and for two times, J23116,I74211for one time and E0240 for seven times (Also made gel control of them). Done the ligation of K517003, I742111 for one time and E0240 for two times. Made the transformation of I742111 and E0240, J23100, B0034, B0015 and parts left for overnight cultere J45700, I74211 for one time and E0240 for four times. We measured the nanodrop values of K517003(1) and K517003(2) as 71,3 ng/µ, 122,7 ng/µ, and we again measured nanodrop values of I74211, E0240, J23116. Made the gel electrophoresis of L, J233116,K517003,I74211,I7211,E0240 and we controlled them to if they contain plasmid by the help of enzymes, EcoR1, Sac1, Not1.Also made gel extraction of parts, J23116,I74211,I74211 and E0240.

 

Week Eighteen (13 July-19 July)

Wet Lab: Done transformation of ligation of A1,A2,A3,C1,C2,C3 and also plasmid isolation of A1,A2,A3, A1.2, 2.1, A2.2, A3.2. We made transformation on A4,A5,B4,B5 and digestion on 1A,2A,3A. Measured nano drop values of A2.2, A3.2, PI4,PI5,PI6,PI7,A4,A5. Done the plasmid isolation of ligations of A4 and A5 and  digestion of PI4,PI6,PI7, A4 and A5 by Not1. Parts left overnight culture of M,N,O,P,R,S and and done their plasmid isolation, nano drop measurements, digestion by Not1 and gel electophoresis.

 

Week Nineteen (20 July- 26 July)

Wet Lab: Comleted digestion of I74211 and gel control and gelextraction of it. Measured nanodrop values of B0034. We studied about wiki, parts were prepared and also prepared four strick plate. Prepared CAD streak plate and plates with Amp., Chl, Kana and empty. Made the digestion of I74211, contolled by gel control of it and the ligation of I74211 and E0240. completed gel extraction of I74211 and transformation of L1 and L2. Colonies were observed on CAD streak plate and made their plasmid isolaton. Then made their digestions and controled by gel and left parts, CAD2 and 5CAD for the overnight culture. From the overnight culture of CAD2 and 5CAD prepared 1-1 glycerol stock. Also compleated plasmid isolation of CAD2, CAD5, L1,L2,L3 and L4.

 

Week Twenty (27 July- 2 August)

Dry Lab: We filled final safety form.  

 

Wet Lab: Took CAD2 and CAD5 from incubator.Measured nanodrop values of CAD2,CAD5, L1.1,L1.2,L1.3,L1,4, also made digestion of these parts and L1. Made gel extraction of parts, CAD2, CAD5 and psB1C and also ligaton of CAD2, CAD5 and E0240. Compleated transformation of L1, B+2,B+5.

 

Week Twenty-one (31 August- 6 September)

Wet Lab: Final parts were prepared for shipment.